Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with lambda-IgLC. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for lambda-IgLC. The competitive inhibition reaction is launched between with pre-coated lambda-IgLC and lambda-IgLC in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of lambda-IgLC in the samples. The color development is stopped and the intensity of the color is measured